Jianxin  Liu , Yaying Ge , Danqing Tian , Zhi Zhang , Huaqiao Ding , Fuquan Shen , Weiyong Wang

Research & Development Centre of Flower, Zhejiang Academy of Agricultural Sciences, Hangzhou, 311202, P.R China
Author    Correspondence author
Plant Gene and Trait, 2012, Vol. 3, No. 1   doi: 10.5376/pgt.2012.03.0001
Received: 05 Nov., 2010    Accepted: 30 Nov., 2011    Published: 05 Dec., 2011

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu et al., 2011, Cloning and sequence analysis of Actin gene from Guzmania, Plant Gene and Trait, Vol.3, No.1 1-5 (doi: 10.5376/pgt.2012.03.0001)

As a house keeping gene, Actin was often used as inner control in quantitative and semiquantitative PCR tests to determine relative expressive amount of target genes. Based on EST monoclones of Actin gene obtained from Guzmania full length cDNA library, its full length cDNA sequence was obtained by primer walking sequencing. The gene, named for Goactin1 (GenBank accession No. HQ184438), consisted of 1 625 bp cDNA sequence, 1 131 bp ORF (open reading frame) which encoded a protein with 377 amino acids residues, and a putative protein which was 41.7 kD at estimated molecular weight, 5.31 at isoelectric point and had a ‘actin superfamily’ conservative domain. The secondary structure of the protein was composed of random coil, alpha helix, extend strand and beta turn by SPOMA analysis. Furthermore, its tertiary structure was also build based on A chain of 2BTF. Through phylogenetic tree analysis, Goactin1 was gathered to the same group with Actin protein in Solanum tuberosum, Gossypium hirsutum, Nicotiana tabacum, Brachypodium sylvaticum, and Arabidopsis thaliana.

Guzmania (Guzmania Ruiz&Pav); Actin; Clone