Zhiying Li ^1,2,3 , Zilin Yi ⁴ , Hanqing Cong ¹ , Li Xu ^1,2,3

1. Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, P.R. China
2. Key Laboratory of South China Gene Resources and Germplasm Creating, Ministry of Agriculture, Danzhou, 571737, P.R. China
3. Key Laboratory of Tropical Crops Germplasm Genetic Improvement and Creation of Hainan Province, Danzhou, 571737, P.R. China
4. Vegetable Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, P.R. China
Author    Correspondence author
Plant Gene and Trait, 2012, Vol. 3, No. 3   doi: 10.5376/pgt.2012.03.0003
Received: 11 Nov., 2011    Accepted: 09 Dec., 2011    Published: 01 Mar., 2012

This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li et al., 2012, Effect of Ca²⁺ Regulators on the CaM Gene Expression in Guzmania ‘Amaranth’ during Flower Induction by Ethylene, Plant Gene and Trait, Vol.3, No.3 13-17 (doi: 10.5376/pgt.2012.03.0003)

Since 1930s, the technology of flowering induction by ethylene in bromeliads has begun to be used in production. So far, it is still effectively used in bromeliads flowering induction. However, There is little clear on the mechanism of ethylene flowering induction. In order to clarify the role of Ca²⁺-CaM system in flowering induction by ethylene in bromeliads, Guzmania ‘Amaranth’ plants were employed to be treated with combination of ethylene and Ca²⁺ accelerator, or Ca²⁺ chelator or Ca²⁺ inhibitor respectively. The expression characteristics of CaM were investigated by the approaches of RT-PCR and Northern blot. The results showed that the Ca²⁺ accelerator can advance the expression peak of CaM at the same time of ethylence treatment, wheresas, Ca²⁺ chelator and Ca²⁺ inhibitor could delay the expression peak of CaM. These results were consistent with the corresponding process of the flowering time, which indicated that the Ca²⁺-CaM system might play an important regulative role in the flowering induction by ethylene in Guzmania ‘Amaranth’, but not a decisive role.
 

Guzmania ‘Amaranth’; Ethylene; Ca²⁺ regulator; CaM; Gene expression