Huaqiao Ding¹ , Lihui Mao¹ , Wei Hu¹ , Qing Dong¹ , Jianxin Liu^1,2

1 Xiaoshan Cotton and Bast Fiber Crops Research Institute of Zhejiang Province, Hangzhou, 311202
2 Yuanpei College, Shaoxing University, Shaoxing, 311200
Author    Correspondence author
Plant Gene and Trait, 2020, Vol. 11, No. 3   doi: 10.5376/pgt.2020.11.0003
Received: 10 May, 2020    Accepted: 22 Jun., 2020    Published: 31 Aug., 2020

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Ding H.Q., Mao L.H., Hu W., Dong Q., and Liu J.X., 2020, Cloning and bioinformatics analysis of chlorophyll degrading gene PPH from Curcuma alismatifolia Gagnep, Plant Gene and Trait, 11(3): 1-8 (doi: 10.5376/pgt.2020.11.0003)

To obtain key enzyme gene PPH in chlorophyll degradation process of Curcuma alismatifolia, on the basis of obtaining a large amount of transcriptome information by sequencing the full-length transcriptome, we had screened and analyzed these transcriptome information and obtained 2 PPH genes which named PPH1 and PPH2. The PPH1 gene (GenBank: MT077178) has a full-length cDNA sequence of 1 795 bp in length, an open reading frame of 1437 bp (from 138 to 1 574 bp), and encode a sequence with 478AA amino acid. The PPH2 gene (GenBank: MT077179) has a full-length cDNA sequence of 1393bp, an open reading frame of 1227bp (from 70 to 1296bp), and encode a sequence with 408AA amino acid. Using Blast, Translate tool (ExPASy), Clustal Omega, Find Conserved Domains (NCBI), ProtParam, TMHMM Server, SOPMA, SWISS-MODEL, ClustalX (1.81), MEGA4.1 and so on. Their amino acid composition, physical and chemical properties, conserved domains, secondary structures, tertiary crystal structures, and molecular phylogeny were predicted and analyzed. The nucleotide and protein amino acid sequences of PPH1 and PPH2 have high homology with these PPH genes of other species, and both of them contain a conserved region PLN02578 with hydrolase characteristic. Molecular phylogenetic analysis showed that a small cluster of PPH1 and PPH2 were closest to Musa acuminate PPH (XP_018677219.1), but far away from dicotyledons. This study provided a molecular basis for improving color of Curcuma alismatifolia sterile bracts by genetic transformation in the future.

Curcuma alismatifolia Gagnep; Chlorophyll; PPH (pheophytinase gene); Gene