Jiangtao Gai , Peng Wang , Xiuxu Ye , Yeyuan Chen

Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, 571101, China
Author    Correspondence author
Plant Gene and Trait, 2021, Vol. 12, No. 4   doi: 10.5376/pgt.2021.12.0004
Received: 23 Nov., 2021    Accepted: 28 Nov., 2021    Published: 08 Dec., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Gai J.T., Wang P., Ye X.X., and Chen Y.Y., Gene identification, evolution and expression of MCT gene in mango (Mangifera indica), Plant Gene and Trait, 12(4): 1-7 (doi:10.5376/pgt.2021.12.0004)

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (MCT) is a key enzyme involved in the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. In order to identify the MCT gene in mango, study the evolutionary route and expression pattern of MCT gene. In this study, with arabidopsis MCT gene as a reference, 14 MCT genes were searched from 12 plant genomes through the BLAST and HMMER. Then, we analyzed the conserved domains, phylogeny, and the expression of MCT gene in mango. The results showed that the MCT protein of mango contains a PF01128.19 domain, and the protein sequences of MCT gene is highly conserved. Phylogenetic analysis showed that the evolution of MCT genes were consistent with the evolutionary route of its species, the number of copies of the MCT genes was tightly controlled from algae to angiosperms. The results of expression analysis showed that the expression of MCT gene in the peels and flesh of developing mango were higher than that in the peels and flesh of mature mango in ‘guire-82’and ‘hongyu’. It is speculated that MCT gene play a more important role in the development of mango. These provide scientific basis for further elucidating the function of MCT gene in mango.

Mango (Mangifera indica); 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (MCT); 2-C-methyl-D-erythritol 4-phosphate (MEP)