Zhang Jie , Xinghao Chen , Dingwei Pang , Anan Duan , Ximan Li , Yuhan Sun , Minsheng Yang

1 Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding; Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants of Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, 100083, China
2 Hebei Provincial Key Laboratory of Forest Germplasm Resources and Forest Protection, College of Forestry, Hebei Agricultural University, Baoding, 071000, China
3 Yunnan Province Key Laboratory of Forest Genetic Improvement and Breeding, College of Forestry, Southwest Forestry University, Kunming, 650224, China
Author    Correspondence author
Plant Gene and Trait, 2022, Vol. 13, No. 1   doi: 10.5376/pgt.2022.13.0001
Received: 13 Jan., 2022    Accepted: 18 Jan., 2022    Published: 27 Jan., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang J., Chen X.H., Pang D.W., Duan A.A., Li X.M., Sun Y.H., and Yang M.S., 2022, Identification of Populus tomentosa OSCA gene family and its expression analysis under salt stress, Plant Gene and Trait, 13(1): 1-10 (doi: 10.5376/pgt.2022.13.0001)

OSCA (Hyperosmdality-gate calcium-permeable channels) is a calcium-permeable cation channel protein. By participating in sensing changes in extracellular osmotic potential, it induces an increase in Ca²⁺ concentration, regulates osmotic potential, and plays an important role in hypertonic stress induction. However, there are few studies on the OSCA genes of poplar. In this study, 16 OSCA genes sequences were identified from Populus tomentosa genome, and renamed them according to their distribution on the chromosomes. Based on phylogenetic tree analysis, the PtOSCAs genes were divided into 4 groups, and the OSCA genes in each group had similar gene structures. Two repetitive events were identified between poplar OSCA family genes, one tandem repetitive event involving 3 genes and a fragment repetitive event involving 2 genes, and the Ka/Ks value of each pair of genes was below 1. Therefore, it can be seen that the OSCA family genes of poplar have a purification effect during evolution. By analyzing the expression of PtOSCAs gene under low and high concentration salt stress, it is found that there are the following three types: Type I, where the expression level increases under both low and high concentration salt stress; Type II, at low and high concentration salt stress, the expression level decreased under high concentration salt stress; Type Ⅲ, the expression level showed an upward trend under low concentration salt stress, but the expression level showed a downward trend under high concentration salt stress. This study provides a reference for the functional analysis of PtOSCAs gene and the genetic improvement of poplar.

Populus tomentosa; OSCA; Salt stress; Expression characteristics