Lingling Li , Yuan Wang , Peijun Zhou , Xuefei Yang , Wenwen Tan , Weibo Sun , Qiang Zhuge

Co-Innovation Center for Sustainable Forestry in Southern China, Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, College of Biology and the Environment, Nanjing Forestry University, Nanjing, 210037, China
Author    Correspondence author
Plant Gene and Trait, 2023, Vol. 14, No. 1   doi: 10.5376/pgt.2023.14.0001
Received: 29 Jan., 2023    Accepted: 03 Feb., 2023    Published: 10 Feb., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li L.L., Wang Y., Zhou P.J., Yang X.F., Tan W.W., Sun W.B., and Zhuge Q., 2023, Cloning and expression analysis of PeDIR19 gene in poplar, Plant Gene and Trait, 14(1): 1-10 (doi: 10.5376/pgt.2023.14.0001)

Dirigent (DIR) gene plays an important role in the formation of lignin and lignans by controlling the stereoselective coupling of pineperin alcohol monomers in plants during the polymerization of lignin and lignans. In this study, we take the ‘Nanlin 895’ (Populus deltoides × P. euramericana cv ‘Nanlin 895’) as the material, and clone the PeDIR19 gene. PeDIR19, the whole length of 588 bp, has a length of 555 bp in ORF region and has only a length of 33 bp intron in the middle. PeDIR19 protein codes 185 amino acids and is a hydrophobic protein. It has four conservative motifs and has a highly conservative structural functional domain between 50 bp ~ 142 bp. PeDIR19 gene was found to have the highest homology with Populus trichocarpa PtDIR19 (Potri.001G009100), with only four bases differences. By phylogenetic analysis, PeDIR19 gene belongs to DIR-B/D subfamily. Through tissue specific expression analysis of ‘Nanlin 895’ with different growth stages, it was found that PeDIR19 gene expression level was the highest in the root during the tissue culture seedlings and in the stem during the pot seedlings. PeDIR19 gene had a strong response after 1 d under the induction of Yangpan Erbaojun (Marssonina brunnea). Under the induction of salicylic acid, methyl jasmonate and abscisic acid stress, PeDIR19 gene can strongly respond to the stress of methyl jasmonate and abscisic acid and weakly respond to the salicylic acid stress. This study provides a solid foundation for further study on the function identification of DIR gene in the biosynthetic pathway of plant resistance.

PeDIR19; Poplar; Expression analysis; Disease resistance