Guangxin Li , Guangyuan Wang , Xiaohui Yu , Qing Mei , Guopeng Wang

Institute of Crop Science, Shanxi Academy of Agricultural Sciences, Taiyuan, 030031, China
Author    Correspondence author
Plant Gene and Trait, 2023, Vol. 14, No. 2   doi: 10.5376/pgt.2023.14.0002
Received: 07 Feb., 2023    Accepted: 14 Feb., 2023    Published: 19 Feb., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Li G.X., Wang G.Y., Yu X.H., Mei Q., and Wang G.P., 2023, Analysis of main characters of rice progenies by injecting sorghum DNA into rice, Plant Gene and Trait, 14(2): 1-8 (doi: 10.5376/pgt.2023.14.0002)

In this study, the whole genome DNA of sorghum apomixes line SSA-1 (Shanxi sorghum apomict-1) was injected into two rice varieties of ‘Liaoyan 28’ and ‘Liaoyan 6’ using the pollen tube pathway method. The genetic characters of the progeny changed obviously. By self-selection and identification, 27 phenotypic stable D₄ generation lines were obtained. Through field planting, the phenotypes of agronomic traits such as heading date, tiller number, plant height, and 1 000-grain weight of the introduced lines were statistically analyzed. The results showed that the agronomic traits of the D₄ generation lines were widely varied, some D₄ generation lines were more inclined to donor sorghum in culm and panicle color, which indicated that the whole genome DNA of sorghum was successfully introduced into the receptor cells, and the expression of the receptor gene was affected. Compared with the recipient parents, the introduced lines showed significant variations in the main traits such as heading date, tiller number, plant height, grain number per ear, grain weight per ear, ear length, and thousand grain weights. Further analysis and selection of mutant lines is helpful to discover new germplasm materials and provide abundant germplasm resources for breeding new rice varieties.

Sorghum apomixes line; Pollen tube pathway method; Main characters; Genetic variation