Xiangyi Luo¹ , Jieming Gao² , Minghui Liu¹ , Dong Cao³ , Yuan Zong^2,3 , Baolong Liu³ , Le Wei¹

1 Qinghai Normal University, Xining, 810008, China
2 Qinghai University, Xining, 810016, China
3 Key Laboratory of Crop Molecular Breeding of Qinghai Province, Northwest Institute of Plateau Biology, Chinese Academy of Sciences, Xining, 810001, China
Author    Correspondence author
Plant Gene and Trait, 2023, Vol. 14, No. 4   doi: 10.5376/pgt.2023.14.0004
Received: 07 Mar., 2023    Accepted: 14 Mar., 2023    Published: 29 Mar., 2023

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Luo X.Y., Gao J.M., Liu M.H., Cao D., Zong Y., Liu B.L., and Wei L., 2023, Bioinformatics analysis of cysteine transmembrane gene CYS in transgenic tobacco, Plant Gene and Trait, 14(4): 1-8 (doi: 10.5376/pgt.2023.14.0004)

Overexpression of MYB transcription factor LrAN2 in tobacco can activate up-regulation of anthocyanin biosynthetic pathways, but the expression of cysteine-rich transmembrane domain protein A (CYS) is significantly reduced. AS an important biological amino acid in plants, the biological research of CYS is rarely reported. In this study, the cloned tobacco CYS gene is 282 bp long and encoding 94 amino acids. The predicted molecular weight and isoelectric point of the encoded protein are 24 019.84 Da and 5.23 respectively, and there are no conserved regions in the sequence. The experimental results show that the protein is hydrophilic, mainly composed of α-helix (19.35%) and irregular coil (65.59%), CYS protein does not pass through the transmembrane region and acts outside the membrane. Phylogenetic tree shows that the CYS gene in tobacco was closely related to the CYS gene in pepper, followed by HTC gene and WIH2 gene in tomato. The results of this study cloned and analyzed the sequence of the coding region of CYS gene to provide a reference for the functional study of CYS gene.

Tobacco (Nicotiana tabacum L.); CYS; Gene cloning; Bioinformatics analysis