Research Report
Regulatory Effects of Nursery Mode and Canopy Closure on the Establishment Survival Rate of Tetrastigma hemsleyanum and Delineation of the Optimal Closure Range 
2 Jiande Forest Farm of Jiande City, Jiande, 311604, Zhejiang, China
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Plant Gene and Trait, 2026, Vol. 17, No. 1
Received: 30 Dec., 2025 Accepted: 30 Jan., 2026 Published: 15 Feb., 2026
To clarify the regulatory factors affecting the establishment survival rate of Tetrastigma hemsleyanum, cutting propagation experiments and field establishment trials were conducted to compare survival performance under different nursery modes and canopy closure conditions. The results demonstrated significant differences in survival rates among the three nursery modes, with container substrate cultivation showing significantly higher survival than direct field cuttings. Establishment survival also differed significantly across canopy closure levels, exhibiting a unimodal response pattern along the closure gradient. Quadratic regression analysis indicated that the predicted maximum survival rate occurred at a canopy closure of approximately 0.67. Based on comprehensive statistical analyses and trend fitting, the optimal canopy closure range for the establishment of T. hemsleyanum was determined to be 0.6–0.7. These findings provide quantitative support for understory cultivation management of T. hemsleyanum.
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. Tetrastigma hemsleyanum
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