Jing Pan , Haoran Wang , Qiang Cheng

College of Forestry, Nanjing Forestry University, Nanjing, 210037, P.R. China
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2022, Vol. 12, No. 1   doi: 10.5376/tgmb.2022.12.0001
Received: 05 Jan., 2022    Accepted: 15 Jan., 2022    Published: 27 Jan., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Pan J., Wang H.R., and Cheng Q., 2022, Cloning and functional analysis of PtATAF1-1 transcription factor gene of Populus trichocarpa, Tree Genetics and Molecular Breeding, 12(1):1-9 (doi:10.5376/tgmb.2022.12.0001)

The ATAF1 protein is a member of the NAC transcription factor family and plays an important regulatory role in plant development and stress response. In this study, using rapid amplification of cDNA ends, a full-length cDNA of ATAF1 homolog was cloned from Populus trichocarpa, and the corresponding gene was named PtATAF1-1. The full-length cDNA sequence of PtATAF1-1 is 1 242 bp, which consists of 3 exons and 2 introns, and an 873 bp coding region. The multiple amino acid sequences alignment showed that PtATAF1-1 protein contains the conserved NAM domain of NAC family. Transient expressing with poplar protoplast shows PtATAF1-1 protein localization in the nucleus. In addition, with the treatment of N-terminal conserved region of bacterial flagellin (Flg22) and abscisic acid (ABA) altered the expression level of PtATAF1-1 gene. In this study, the homologous cloning and functional analysis of the PtATAF1-1 gene were performed, which provided a reference for the further development of the function of ATAF1 gene in poplar.

Populus trichocarpa; PtATAF1-1 gene; Expression analysis; Subcellular localization