Weifeng Liu , Jiayin Hou , Xiangning Jiang

College of Biology, Beijing Forestry University, Beijing, 100083, China
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2022, Vol. 12, No. 2   doi: 10.5376/tgmb.2022.12.0002
Received: 09 Feb., 2022    Accepted: 15 Feb., 2022    Published: 18 Mar., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu W.F., Hou J.Y., and Jiang X.N., 2022, Optimization of somatic embryo regeneration of Larix olgensis through multiple media, Tree Genetics and Molecular Breeding, 12(2): 1-11 (doi:10.5376/tgmb.2022.12.0002)

To establish a stable and efficient Larix olgensis somatic embryo regeneration system. In this study, immature zygotic embryos of Larix olgensis were used as explant materials for embryogenic callus induction, and embryogenic callus with good growth were selected to induce somatic embryo formation by liquid medium A, semi-solid medium B, and solid medium C. The callus induction medium is S medium+2,4-D 1.0 mg/L + 6-BA 0.5 mg/L + KT 0.3 mg/L, sucrose 20 g/L, agar 3 g/L; the subculture medium is S medium+2, 4-D 0.15 mg/L + 6-BA 0.05 mg/L + KT 0.05 mg/L, sucrose 30 g/L, agar 6 g/L. Mature medium A is S medium + PEG4000 100 g/L + ABA 15 mg/L + AgNO₃ 10 mg/L, sucrose 45 g/L liquid medium. Mature medium B and C are mature medium A supplemented with 2 g/L and 10 g/L agar, respectively. The maturation time of this method is about 32 days, the synchronization rate is 78.0%, and the proportion of somatic embryos in normal morphology is 83.0%. Somatic embryo formation efficiency in normal morphology can reach 223.0 embryos/g embryogenic callus, somatic embryo germination rate can reach 47.2%. Compared with a single maturation medium, the maturation time is shortened, somatic embryo generation is more synchronized, and high somatic embryo yield and quality are improved. It provides a way for the commercialization of somatic embryo regeneration of Larix olgensis, brings great convenience to large-scale breeding and reproduction, physiological and biochemical research, and genetic modification, and has great practical application value.

Larix olgensis; Embryogenic callus; Somatic embryogenesis