Tingting Yang^1,2 , Zhi Feng¹ , Yanling Liu¹ , Guo Yang¹ , Meng Li¹ , Yiqiang Wang^1,2

1 Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China
2 Key lab of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha, 410004, China
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2022, Vol. 12, No. 3   doi: 10.5376/tgmb.2022.12.0003
Received: 17 Feb., 2022    Accepted: 24 Feb., 2022    Published: 18 Mar., 2022

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Yang T.T., Feng Z., Liu Y.L., Yang G., Li M., and Wang Y.Q., 2022, Transcriptome analysis and differential gene screening of male flower buds in three age stages of Ginkgo biloba, Tree Genetics and Molecular Breeding, 12(3): 1-9 (doi: 10.5376/tgmb.2022.12.0003)

In this paper, the key genes of flowering regulation among three age stages was screened and the molecular mechanism of the flowering pathway was revealed in Ginkgo biloba. It can provide scientific basis for promoting early flowering, molecular breeding and spreading planting of Ginkgo biloba. High throughput sequencing technology and bioinformatics tools were used to sequence the transcriptome of male flower buds of Ginkgo biloba in three age stages. By analyzed the sequencing data, we screened out the key differential expression genes of age pathway. A total of 57.45 Gb of raw data was generated by transcriptome sequencing. The total number of Unigenes was 35 058 and annotated in the 8 functional databases (GO, COG, KEGG, KOG, NR, Pfam, Swiss-Prot, and eggNOG). The Unigenes were classified into 55 GO categories and 126 metabolic pathways. Analysis of differentially expressed genes revealed that 37 genes were up-regulated and 75 genes were down-regulated during the flower bud undifferentiated stage vs. initial stage of flower bud differentiation. A total of 592 genes were up-regulated and 871 genes were down-regulated during the initial stage of flower bud differentiation vs. flower bud differentiation stage. The flower bud undifferentiated stage vs. flower bud differentiation stage，961 genes were up-regulated and 1 203 genes were down-regulated. A large number of flowering related genes were discovered and finally screened out 11 key genes of flowering regulation in age pathway. including SPL(gene.Gb_23724, gene.Gb_03922), AP2(gene.Gb_00766), MADS-box(gene.Gb_01886, gene.Gb_15398, gene.Gb_28337, Gingko_newGene_2213), Gibberellin-regulated protein(gene.Gb_34467, gene.Gb_28606, gene.Gb_33214)and DELLAs protein(gene.Gb_34644).

Ginkgo biloba; Transcriptome sequencing; Age pathway; Differentially expressed genes