Feature Review

Cloning and Functional Analysis of Key Genes Involved in Anthocyanin Biosynthesis in Morella rubra  

Zhen Liu1 , Dandan Huang2 , Wenfang Wang1
1 Institute of Life Sciences, Jiyang Colloge of Zhejiang A&F University, Zhuji, 311800, Zhejiang, China
2 Hainan Institute of Biotechnology, Haikou, 570206, Hainan, China
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2025, Vol. 15, No. 3   
Received: 17 Apr., 2025    Accepted: 19 May, 2025    Published: 30 May, 2025
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

This study reviews the research achievements made in recent years regarding the synthesis of anthocyanins in Morella rubra. Key genes such as CHS, CHI and F3H play an important role in the synthesis process of anthocyanins. In addition, some factors that regulate these genes, such as R2R3-MYB, bHLH and WD40, also play a role in the regulatory process. There is also a mutually cooperative relationship among them. To figure out how these genes work, researchers employed methods such as cloning genes, analyzing expression patterns, and also studied the epigenetic regulation of genes. This study also introduces the application prospects of molecular breeding, genetic engineering and CRISPR technology in improving the color and nutritional value of Morella rubra. It is hoped that these achievements can provide a scientific basis for improving the quality of Morella rubra and also bring practical methods for planting and breeding.

Keywords
Morella rubra; Anthocyanin biosynthesis; MYB transcription factor; Molecular breeding; Epigenetic regulation
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