Rajesh Pati ^1,2 , Maneesh Mishra ² , Ramesh Chandra ² , Muthukumar M. ²

1 Jain R&D, Jain Irrigation Systems Ltd., Jalgaon-425001, Maharashtra, India;
2 Central Institute for Sub-tropical Horticulture, Rehmankhera, Lucknow-227107, India
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2013, Vol. 3, No. 3   doi: 10.5376/tgmb.2013.03.0003
Received: 18 Mar., 2013    Accepted: 31 Mar., 2013    Published: 27 Apr., 2013

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Pati et al., 2013, Histological and biochemical changes in Aegle marmelos Corr. before and after acclimatization, Vol.3, No.3 12-18 (doi: 10.5376/tgmb. 2013.03.0003)

Maximum mortality of micropropagated plants occur during acclimatization phase because plantlets undergo rapid and extreme changes in physiological functioning, histological and biochemical changes. In order to investigate the actual reason of this limitation, test samples were collected at different stages of micropropagation of Aegle marmelos Corr. (in vitro stage , acclimation stage, field established plants). The biochemical result showed that micropropagated plantlets produced significantly low total chlorophyll (0.042 mg/g fresh weight), reducing sugar (3.227%), NR activity (1.353 NO₂/h/g fresh weight) and but higher protein (0.048 µg/g) during in vitro phase. The in vitro raised plants showed abnormal histological features like altered leaf mesophyll, absence of thick cuticle, sunken stomata, poorly developed stem and root histology. These results revealed that the photoautrophic mode of nutrition during in vitro phase increased the survival rate during acclimatization compared to photoheterotrophic mode of nutrition. This suggests that photoautotrophism phenemoneon has substantial influence on the physiology and development of in vitro regenerated Aegle marmelos Corr. plantlets.

Histology; Parenchymous cells; Chlorophyll; Micropropagation and stomata