QTLs Location of Calyx-related Traits in Tetraploid Dendrobium
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, The Engineering Technology Research Center of Tropical Ornamental Plant Germplasm Innovation and Utilization, Hainan Province, Danzhou, 571737, China
Molecular Plant Breeding, 2019, Vol. 10, No. 6
Received: 11 Mar., 2019 Accepted: 25 Mar., 2019 Published: 29 Mar., 2019
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This article was first published in Molecular Plant Breeding (2018, 8: 2533-2539) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In order to identify QTL locus of calyx-related traits in Dendrobium, 190 F1 individuals derived from the crossbreed of tetraploid Dendrobium cultivars D. Mangosteen x D. Burana Pink No.2 were used as materials. Through three years observation of four characteristics of calyx (length of dorsal sepal, width of dorsal sepal, length of lateral sepal, width of lateral sepal), the QTL was located on the two genetic maps of parents which had been already constructed. The result showed that the four characteristics were quite different among the individuals of F1 generation, and the observed values of all the characteristics were all positive distribution, which was suitable for QTL analysis. Nine QTLs were detected on the maternal ‘D. Mangosteen’ genetic linkage map, including 1 QTL for dorsal sepal length, 3 QTLs for dorsal sepal width, 2 QTLs for lateral length and 3 QTLs for lateral width, with the genetic contribution rate ranging from 11.9% to 16.8%. Also, two tight linkage markers were acquired (M10E3-146, M8E8-284). A total of 6 QTLs were detected on the paternal ‘D. Burana Pink No.2’ linkage map, including 2 QTLs for dorsal sepal length, 1 QTL for dorsal sepal width, 2 QTLs for lateral length and 1 QTL for lateral width, with the genetic contribution rate ranging from 11.8% to 17.3%. This result of study could provide reference for Dendrobium molecular marker assisted breeding and precise location of correlation gene.
Tetraploid Dendrobium; Calyx-related traits; QTL location