Research Report

Cloning and Expression Analysis of atp6 Genes between Cytoplasmic Male Sterile Line and its Maintainer Line in Bunching Onion  

Qinghua Wang1 , Rongting Zhang2 , Shujuan Jia3 , Limin Gao1
1 Vegetable and Flower Research Institute of Shandong Academy of Agricultural Sciences/ Shandong KeyLaboratory of Greenhouse Vegetable Biology / Shandong Branch of National Vegetable Improvement Center / Vegetable Science Observation and Experimental Station in Huang-Huai District of Ministry of Agriculture, Jinan, 250100
2 Jinan Institute of Agricultural Sciences, Jinan, 250316
3 Jiyuan Agricultural Comprehensive Development Office, Jiyuan, 459000
Author    Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 25   
Received: 12 Oct., 2020    Accepted: 13 Oct., 2020    Published: 13 Oct., 2020
© 2020 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding ( ISSN1672-416X,CN46-1068/S) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
In order to further study the relationship between atp6 gene and Cytoplasm male sterility (CMS) of bunching onion, the sequence of atp6 gene was obtained from male sterile line and maintainer line of Zhangqiu onion bunching using homologous cloning. The protein secondary structure and the expression of atp6 gene in different growth stages were compared. The results showed that there was one polymorphic locus (A/T) at the 171st base of the open reading frame of atp6 gene in CMS line and maintainer line, which changed the proportion of protein secondary structure slightly, but did not change the transmembrane structure of ATP6 protein. The expression of atp6 gene in roots and leaves/flowers at seedling, bolting, flowering and late flowering stage was detected by real-time fluorescence quantitative polymerase chain reaction.The results showed thatthe expression of atp6 gene in root showed a trend of increasing at first and then decreasing during the whole development of the plant, reaching the maximum at the flowering stage, and the expression of the maintainer line was 1.14 times higher than that of the CMS; The expression of atp6 gene in leaves/flowers decreased gradually during the whole growth period, and reached the highest level at the seedling stage, and the maintainer line was 1.96 times higher than that of the CMS.
Keywords
Bunching onion; atp6 gene; Cloning; Expression analysis

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Molecular Plant Breeding
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. Qinghua Wang
. Rongting Zhang
. Shujuan Jia
. Limin Gao
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