Gang Guo¹ , Jing Yu² , Degang Zhao^1,3

1. Guizhou Key Laboratory of Agro-Bioengineering, College of Life Sciences, Guizhou University, Guiyang, 550025, P.R. China
2. Guizhou Tongren flue-cured tobacco limited liability company, Tongren, 554300, P.R. China;
3. Guizhou Key Laboratory of Green Pesticide and Agricultural Biological Engineering, Ministry of Education, Guiyang, 550025, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2011, Vol. 2, No. 2   doi: 10.5376/mpb.2011.02.0002
Received: 13 Jul., 2010    Accepted: 23 Nov., 2010    Published: 05 Jan., 2011

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Guo et al., 2011, Rapid acquirement of transgenic rice plants derived from callus of mature embryos transformed by agrobacterium mediation, Molecular Plant Breeding, Vol.2 No.2 (doi: 10.5376/mpb.2011.02.0002)

In this research we transformed calli of two elite germplasms, Taipei 309 and Nipponbare, derived from mature embryos using the construct of pCAMBIA0390 mediated by Agrobacterium tumefaciens strain EHA105. The expression plasmid pCAMBIA0390 was constructed with expressing elements of ubi:bar-Gus and ACT:Bt. Meanwhile We studied the effects of the factors on the regeneration rates of callus tissues. The results showed that the regeneration frequencies of Taipei 309 and Nipponbare increased up to 39% and 44% respectively, under the procedures as following: Callus was cultured 21days in the conditions of, 28℃, 2000 Lx, and 13 h/d illumination period. Infected callus air-dried 20 minutes and then co-cultured callus air-dried 20 minutes, finally, the callus desiccated using sterile filter paper of callus 8 hours. It shows that transformation cycle can shortened from 105 days to 60 days in absences of subculture stage. The GUS expression and PCR analysis indicated that the exogenous Bt gene has been integrated into the rice genome. 

Rice;Mature embryos;Rapid regeneration;Transformation mediated by Agrobacterium