Differential expressed genes of banana leaves induced by Fusarium wilt disease 
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Chen Y.Y. ,
Luo J.Y. ,
Zhao H. ,
Mu L. ,
Xie J. ,
Wei S.S. ,
Xia Y.Q. ,
Tang H. 
Author Correspondence author
Molecular Plant Breeding, 2015, Vol. 6, No. 20 doi: 10.5376/mpb.2015.06.0020
Received: 25 Aug., 2015 Accepted: 26 Sep., 2015 Published: 08 Nov., 2015
Peng T.C., Chen Y.Y., Luo J.Y., Zhao H., Mu L., Xie J., Wei S.S., Xia Y.Q. and Tang H., 2015, Differential expressed genes of banana leaves induced by Fusarium wilt disease, Interaction, Molecular Plant Breeding, 6(20): 1-10 (doi: 10.5376/mpb.2015.06.0020)
Fusarium wilt of banana is a destructive fungus disease caused by Fusarium oxysporum f. sp. Cubense. This disease had harmful effects on banana planting and its industrial production. In order to study the pathogenic mechanism of banana Fusarium wilt disease, we used cDNA-AFLP technique to identify differential expressed transcripts that were induced or inhibited by Fusarium oxysporum f. sp. Cubense tropical race 4 infection. Among 223 isolated cDNA fragments, we sequenced and get 137 unique banana cDNAs that involved in different functions, including disease and defense response, transcription regulation, signal transduction, primary metabolism, energy metabolism, cell growth and division, protein destination and storage, and so on. Among these genes, 8 ESTs were related to disease and defense response including NBS-LRR type resistance gene, hot shock protein, and alcohol dehydrogenase; 19 ESTs were related to transcriptional regulation including transcription factor MYB and MYC, zinc finger protein, and Glycine-rich RNA binding protein; 12 ESTs were related to signal transduction including the Ras-related nuclear proteins. We choose out 6 important genes to analyze the expression patterns by sqRT-PCR, and confirm their relationship with pathogenic mechanism of banana Fusarium wilt.