Xiao Shuiping^1,2 , Wang Kunbo² , Yang  Lei¹ , Ke  Xingsheng¹ , Wang  Chunying² , Liu  Xinwen¹ , Sun  Liangqing¹ , Yang  Shaoqun¹ , Liu  Fang² , Chen Yi¹

1 Cotton Research Institute of Jiangxi Province/ Poyang Lake Cotton Experiment Station, CARS, Jiujiang, Jiangxi 332105, China
2 Institute of Cotton Research of CAAS / State Key Laboratory of Cotton Biology, Anyang, Henan, 455000, China
Author    Correspondence author
Molecular Plant Breeding, 2016, Vol. 7, No. 14   doi: 10.5376/mpb.2016.07.0014
Received: 12 Jan., 2016    Accepted: 27 Feb., 2016    Published: 04 Apr., 2016

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Xiao S.P., Wang K.B., Yang L., Ke X.S., Wang C.Y., Liu X.W., Sun L.Q., Yang S.Q., Liu F., and Chen Y., 2016, Analyses on Progenitor Donors of the Cultivated Allotetraploid Cottons Revealed by GISH, Molecular Plant Breeding, 7(14): 1-10 (doi: 10.5376/mpb.2016.07.0014)

GISH (Genomic in situ hybridization) of the mitotic metaphase chromosomes of two cultivated tetraploid cotton (AD)₁ (G. hirsutum) and (AD)₂ (G. barbadense) with all 3 diploid A cotton gDNA(genomic DNA) as probes, blocking with ssDNA(salmon sperm DNA) respectively. The hybridization signals were dected distribute in the A sub-genome chromosomes of (AD)₁ and (AD)₂, besides, three pairs of crimson signals were also detected only with the A_1-a gDNA probe. which were named GISH-NORs. GISH of (AD)₁ and (AD)₂ with all 13 diploid D cotton gDNA as probes, blocking with ssDNA respectively, except the D₆ (G.gossypiodies) gDNA probe generated the hybridization signals in all the chromosomes of (AD)₁ and (AD)₂, the other 12 diploid D gDNA probes only generated the signals on the D sub-genome chromosomes of (AD)₁ and (AD)₂, the D6 gDNA probe was very specifical.And three pairs of strong GISH-NORs were detected with all 13 diploid D genome species gDNA probes, the intensity of their GISH-NORs were much brighter than the A_1-a gDNA probe. These results visually confirmed the amphidiploid origin of the allotetraploid cotton species. DA (distinguishing ability ) values of each gDNA probe generated were calculated basing on the above GISH rsults.It showed that the DA value of A_1-a gDNA probe was the biggest in all 3 diploid A genomes both in (AD)₁ and (AD)₂ GISH, and this indicated that A_1-a genome was most likely to be the A sub-genome progenitor donor of (AD)₁ and (AD)₂, while the D_3-d (G. davidsonii ) and D₅ (G. raimondii) genome species were most likely to be the D sub-genome progenitor donor of (AD) ₁, and (AD) ₂ respectively. And this further confirmed that tetraploid cottons are polyphyletic.

Gossypium; G.hirsutum L.; G.barbadense L.; Progenitor; GISH; GISH-NOR