Pengyu Zhang¹ , Guorui Wang¹ , Xiao Qiu¹ , Baona Zhang¹ , Li Wei^1,2 , Tongchao Wang¹

1 The Collaborative Innovation Center of Henan Grain Crops, Henan Agricultural University, Zhengzhou, 450046, China
2 The National Engineering Research Centre for Wheat, Henan Agricultural University, Zhengzhou, 450046, China
Author    Correspondence author
Molecular Plant Breeding, 2020, Vol. 11, No. 2   doi: 10.5376/mpb.2020.11.0002
Received: 23 Apr., 2020    Accepted: 14 May, 2020    Published: 19 Jun., 2020

This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Zhang P.Y, Wang G.R., Qiu X., Zhang B.N., Wei L., and Wang T.C., 2020, Functional analysis of Tadhn1312 by virus-induced gene silencing (VIGS) in common wheat (Triticum aestivum L.), Molecular Plant Breeding, 11(2): 1-9 (doi: 10.5376/mpb.2020.11.0002)

Virus-induced gene silencing (VIGS) is an important tool for gene function analysis in plants. In the present study, the function identification of a candidate gene named Tadhn1312 encoding Dehydrin protein obtained from the high-throughput transcriptome was carried out by VIGS. Leaves appeared the chlorotic phenotype and the transcript level of TaPDS decreased rapidly at 7 days after the inoculation by BSMV: TaPDS, which indicated that the virus had successfully infected wheat leaves and the BSMV system was efficient. The chlorophyll content of leaves with BSMV: TaPDS and BSMV: Tadhn1312 inoculation decreased at 7 days, and reached significant level compared with the control. After the inoculation by BSMV: Tadhn1312, the transcript level of Tadhn1312 was rapidly decreased at 7 days, and reached the minimum value at 21 days, indicated that Tadhn1312 had been silenced. The spike differentiation procession of wheat plants inoculated by BSMV: Tadhn1312 was late than that inoculated by BSMV: 00. The result showed that the silencing of Tadhn1312 prolonged the spike differentiation process, illustrating that Tadhn1312 gene was involved in the spike differentiation process of wheat directly or indirectly.

Wheat; Gene silencing; BSMV; Spike differentiation