Hao Wu¹ , Guofu Deng¹ , Lijun Gao¹ , Gaoxing Dai² , Juan Huang¹ , Ju Gao¹ , Dongjin Qing¹ , Weiwei Chen² , Shuangyong Yan³ , Haifu Liang²

1 Guangxi Crop Genetic Improvement and Biotechnology Laboratory, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
2 Rice Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, China
3 Tianjin Key Laboratory of Crop Genetics and Breeding, Tianjin Crop Research Institute, Tianjin, 300384, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 23   doi: 10.5376/mpb.2021.12.0023
Received: 16 Jul., 2021    Accepted: 19 Jul., 2021    Published: 25 Jul., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Wu H., Deng G.F., Gao L.J., Dai G.X., Huang J., Gao J., Qing D.J., Chen W.W., Yan S.Y., and Liang H.F., 2021, Development and breeding application of the fluorescence molecular marker of rice bacterial blight resistance gene Xa7, Molecular Plant Breeding, 12(23): 1-8 (doi: 10.5376/mpb.2021.12.0023)

In this study, the closely linked fluorescence molecular marker PM-Xa7 of Xa7 gene was developed on the basis of sequence difference between the resistant variety ‘IRBB7’ containing Xa7 and other nine bacterial blight susceptible rice varieties, and combine with PARMS technology (Penta-primer amplification refractory mutation system). Genotypes of Xa7 were identified in 72 rice germplasm and the bacterial blight resistance level of ‘Mei B’ was improved by backcross and screening with this marker. The results showed that two rice germplasms containing Xa7 were screened out from 72 rice germplasm; By introducing the resistant Xa7 gene from ‘IRBB7’ into ‘Mei B’ with PM-Xa7, a stable new maintainer with resistance to bacterial blight was successfully obtained. The bacterial blight resistant fluorescence signal was detected in 289 plants and the bacterial blight susceptible fluorescence signal was detected in 93 plants of BC₂F₂population generated from ‘IRBB7’ and ‘Mei B’, and the genotypes were consistent with the phenotypes of Xoo inoculation. These research results indicated that PM-Xa7 can be effectively used for the marker-assisted breeding of bacterial blight resistant rice variety with the resistant Xa7 gene.

Rice; Bacterial blight; Xa7; Fluorescence molecular marker