Meng Tan¹ , Yuxin Shi¹ , Haisu Zheng^1,2 , Liu Shao^1,2 , Peimin He^1,2

1 College of Marin Ecology and Environment, Shanghai Ocean University, Shanghai 201306, China
2 Water Environment & Ecology Engineering Research Center of Shanghai Institution of Higher Education, Shanghai 201306, China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 30   doi: 10.5376/mpb.2021.12.0030
Received: 24 Sep., 2021    Accepted: 30 Sep., 2021    Published: 09 Oct., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Tan M., Shi Y.X., Zheng H.S., Shao L., and He P.M., 2021, Development of polymorphic SSR markers in Vallisneria based on RAD-seq, Molecular Plant Breeding, 12(30): 1-7 (doi:10.5376/mpb.2021.12.0030)

Vallisneria belongs to Hydrocharitaceae, which is a pioneer species of ecological restoration in waters. In order to carry out a more comprehensive SSR analysis ofVallisneria, we used the restriction-site associated DNA sequencing (RAD-seq) technology to analyze Simple Sequence Repeats (SSR) information of Vallisneria, SSR markers and primer of Vallisneria were developed based on RAD-seq data．Among which ,366 simple sequence repeats (SSR) loci were detected. Primers of 355 loci were designed successfully, and the two base repeat type SSR loci were the largest (59.56%). Finally, we confirmed 23 pairs of polymorphic SSR primers after the screening and validation. The analysis results by Genepop software showed that the mean number of alleles of the 23 loci is 3.26, and these loci do not link to each other (P<0.01). Four loci deviate from HWE (P<0.01) and the number of heterozygous roughly the same as that of homozygotes (observed heterozygosity mean of 0.460), inbreeding coefficient is high (mean of 0.880). Which could be caused by take sample too closely in the same sampling, andVallisneria have a combination of generative propagation and clone.

Vallisneria; RAD-seq; SSR molecular marker; Reduced-representation genome sequencing