Xiaocong Cao^1,2 , Chaojun Zhang² , Haoqi Gou² , Xiaoyan Wang³ , Kaikai Qiao² , Qifeng Ma² , Guiyin Zhang¹ , Shuili Fan^1,2

1 Hebei Agricultural University, Hebei Base of state Key Laboratory of Cotton Biology, Baoding, 071001, P.R. China
2 Institute of Cotton Research of the Chinese Academy of Agricultural Sciences, State Key Laboratory of Cotton Biology Cotton, Anyang; 455000, P.R. China
3 Anyang Institute of Technology, Anyang, 455000, 130033, P.R. China
Author    Correspondence author
Molecular Plant Breeding, 2021, Vol. 12, No. 27   doi: 10.5376/mpb.2021.12.0027
Received: 15 Sep., 2021    Accepted: 25 Sep., 2021    Published: 21 Oct., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Cao X.C., Zhang C.J., Gou H.Q., Wang X.Y., Qiao K.K., Ma Q.F., Zhang G.Y., and Fan S.L., 2021, Genome-wide identification and analysis of β-galactosidase (BGAL) gene family in cotton, Molecular Plant Breeding, 12(27): 1-16 (doi: 10.5376/mpb.2021.12.0027)

Plant β-galactosidases (BGALs) are the important glycosidase that hydrolyses the non-reductive terminaβ-D-galactosidase residues from glycochains, glycolipids and glycoproteins. In order to reveal the regulation mechanism of β-galactosidases in cotton development, the whole genome analysis of BGAL gene family were carried out in this study, which laid a foundation for further understanding of the function of BGAL genes in cotton pollen. A total of 153 BGAL genes were respectively identified in Gossypium hirsutum, G.barbadense, G.arboreum, G.raimondii. Phylogenetic tree analysis showed that the BGAL genes are divided into eight subgroups, with the same number of exons and genetic structure in the same subgroup. The co-evolutionary analysis showed that there were multiple gene pairs between G.hirsutum and diploid cotton. The transcriptomic data showed that some genes in G.hirsutum were specifically expressed in different tissues. For example, GhBGAL6 and GhBGAL32, are highly expressed in all tissues; GhBGAL33, GhBGAL7, GhBGAL18 and GhBGAL43 were highly expressed in stamens (anther and filament) and petals. Further qRT-PCR results showed that some genes (such as GhBGAL6, GhBGAL7 and GhBGAL17) were highly expressed in stamens, which may have a certain regulatory effect on the development of stamens. This study explored the evolution and function of the BGAL gene family in the genomes of cotton, which can provide a theoretical basis for subsequent research on BGAL genes in cotton.

β-galactosidases; Bioinformatics; Cotton; Gene family