Research Article
Genomic Variation Detection Analysis of 3 Self-pruning Tomato Lines Based on Resequencing
2 Hebei Key Laboratory of Horticultural Germplasm Excavation and Innovative Utilization, Hebei Normal University of Science & Technology, Qinhuangdao, 066004, China
Author Correspondence author
Molecular Plant Breeding, 2022, Vol. 13, No. 9 doi: 10.5376/mpb.2022.13.0009
Received: 15 Mar., 2022 Accepted: 21 Mar., 2022 Published: 31 Mar., 2022
Du H.D., You X., Li Y.F., Wang S., Mao X.J., and Zhang N., 2022, Genomic variation detection analysis of 3 self-pruning tomato lines based on resequencing, Molecular Plant Breeding, 13(9): 1-8 (doi: 10.5376/mpb.2022.13.0009)
A close relationship was indicated between the yield of self-pruning tomato varieties and the number of main stem flowers. There were many reports on the genes controlling the traits of inflorescence sealing, but few on the related studies controlling the nodes of inflorescence sealing. To study the genetic variation between different self-pruning tomatoes stem and inflorescence segments, the candidate genes involved in the regulation of inflorescence segments were screened out. In this experiment, the variation of 3 tomatoes strains GXF, AXF, and 815 were detected by resequencing technology. The results showed that a total of 5 968 501 SNPs and 485 114 Indel were detected in the three samples, and a total of 33 473 gene mutations were detected after comparison with the reference genome. The GO and KEGG databases were used to compare the mutations in the CDS region, and it was found that they mainly focused on basic metabolism and zein biosynthesis. Through genome sequence alignment analysis, 16 genes that may be involved in the regulation of the terminal segments of the tomato main stem were screened. The results confirmed the mutation locus information of related genes and laid a foundation for further research on the genetic mechanism of inflorescence positions of self-pruning tomatoes.