Research Article

Effect of Sodium Nitroprusside on the Growth and Enzymes Related to Nitrogen Metabolism of Melon Seedlings under Low Temperature Stress  

Yongping Zhang1 , Qiannan Diao1 , Yanyan Cao1 , Mengya Shi2 , Hongmei Zhang1 , Haijun Jin1 , Shouyang Jiang3 , Haibin Shen1
1 Horticultural Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai Key Laboratory of Protected Horticultural Technology, Shanghai, 201403, China
2 National Agro-Tech Extension and Service Center, Beijing, 100125, China
3 Shanghai Agricultural Scientific Seed and Seeding CO., LTD, Shanghai, 201106, China
Author    Correspondence author
Molecular Plant Breeding, 2023, Vol. 14, No. 9   doi: 10.5376/mpb.2023.14.0009
Received: 10 May, 2023    Accepted: 15 May, 2023    Published: 22 May, 2023
© 2023 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang Y.P., Diao Q.N., Cao Y.Y., Shi M.Y., Zhang H.M., Jin H.J., Jiang S.Y., and Shen H.B., 2023, Effect of sodium nitroprusside on the growth and enzymes related to nitrogen metabolism of melon seedlings under low temperature Stress, Molecular Plant Breeding, 14(9): 1-8 (doi: 10.5376/mpb.2023.14.0009)


In order to study the effects of 200 μmol/L SNP for 1, 3, 5, 7 d on the growth and the enzymes related to nitrogen metabolism under low temperature stress 12/6℃, day/night, and to explore the feasibility of exogenous SNP regulating low temperature stress on melon (Cumumis melo. L) seedlings, the melon seedlings were used as experimental materials and cultivated by substrate culture method. NO donor sodium nitroprusside (SNP) was sprayed on the leaves. The results showed that with the prolongation of low temperature stress, the seedlings growth and nitrogen metabolism were inhibited to some extent. After treatment with exogenous SNP , the seedlings growth (leaf area, shoot fresh weight and root fresh weight) , the contents of nitrate nitrogen, free amino acid, soluble protein and the activities of key enzymes of nitrogen metabolism (NR, NiR, GS, GDH, GOGAT and AS) were all greatly improved; the contents of ammonium nitrogen, the accumulation of H2O2 and the production rate of superoxide anion radical (O2-) decreased dramatically. In summary, the application of exogenous SNP can effectively alleviate the inhibition of low temperature stress on the growth and nitrogen metabolism, and enhance its low temperature tolerance. The longer the stress, the more obvious the effect.

Melon; Low temperature stress; Sodium nitroprusside; Nitrogen metabolism
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. Yongping Zhang
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. Haibin Shen
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