Jing Xu¹ , Xue Han¹ , Xiaogen Pan² , Jiliang Pang¹

1. College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, 310036, P.R. China
2. Realty Service Center, Hangzhou Normal University, Hangzhou, 310036, P.R. China
Author    Correspondence author
Plant Gene and Trait, 2011, Vol. 2, No. 1   doi: 10.5376/pgt.2011.02.0001
Received: 22 Nov., 2011    Accepted: 08 Dec., 2011    Published: 17 Dec., 2011

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Xu et al., 2011, Transformation of Cucumber Flowering Integrator (CFL Gene) into Cyclamen persicum Mediated by Agrobacterium, Molecular Plant Breeding, 9(5): 629-634 (doi: 10.3969/mpb.009.000629)

By employing cyclamen young leaves, petioles and callus from Cyclamen persicum as explants, transformation of cucumber flowering integrator (CFL gene) into the genome of Cyclamen persicum mediated by Agrobacterium tumefaciens was conducted to compare the transformation efficiencies of different explants and to optimize the sonication-assisted transformation procedure mediated by Agrobacterium. The results demonstrated that the CFL gene was integrated into the genome of regenerated Cyclamen plants, which were validated by PCR and RT-PCR and by GUS histochemical assay. The results also showed that young leaves were the best receptor for transformation among employed explants, which the GUS-positive rates of regenerated plants can reach 27.25%. Furthermore, GUS positive rate can be increased four times by using the sonication-assisted transformation procedure. In this study, we successfully introduced the CFL gene into the genome of Cyclamen persicum by using modified high-efficiency genetic transformation system, which might be an alternative way for developing early flowering Cyclamen variety.
 

Cyclamen persicum; Cucumber flowering integrator; CFL gene; Agrobacterium-mediated transformation; Sonication-assisted transformation