Shenping Xu^1,2 , Xiaorong Liu¹ , Jingmei Liu¹ , Guoping Wang³ , Fangqing Lian² , Feixiong Liao¹

1. Floricultural Research Institute, Guangdong Academy of Agricultural Science, Guangzhou, 510640, P.R. China
2. College of Landscape and Art, Jiangxi Agriculture University, Nanchang, 330045, P.R. China
3. College of Horticulture, South China Agricultural University, Guangzhou, 510640, P.R. China
Author    Correspondence author
Plant Gene and Trait, 2012, Vol. 3, No. 7   doi: 10.5376/pgt.2012.02.0007
Received: 09 Mar., 2012    Accepted: 20 Jun., 2012    Published: 27 Jun., 2012

This article was first published in Molecular Plant Breeding (2011, 9(1): 104-112) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Xu et al., 2012, Analysis of QTL for Three Leaf Traits in Phalaenopsis based on AFLP Molecular Genetic Map, Plant Gene and Trait, Vol.3, No.7 34-42 (doi: 10.5376/pgt. 2012.03.0007)

 

In this research, we employed a hybrid population between ‘Phal.462’ and ‘Phal.20’as the tested materials to evaluate the leaf length (LL), leaf width (LW), plant width (PW) of 88 individual Phalaenopsis progenies. These 3 traits of the progenies showed continuous normal distribution statistically in the population, and a significant correlation was found between LL and PW. The quantitative trait locus (QTL) controlling those traits were analyzed by using interval mapping method based on the AFLP marker linkage maps. The results showed that a total of 60 QTLs covered eight linkage groups were detected in the paternal parent genetic map, 20 of which was for LL, 19 for LW and 21 for PW. Phenotypic variation explained by each QTL ranged from 44.1% to 89.4% with the LOD value from 3.05 to 14.78. While a total of 28 QTLs were detected on five linkage groups of the maternal parent genetic map, 10 of which was for LL, 8 for LW and 10 for PW. Phenotypic variation explained by each QTL ranged from 57.7% to 76.3%, with the LOD value from 3.11 to 9.24. This may be the first QTL analysis in Phalaenopsis in the world as the authors knew, which could provide a new tool for cloning of genes and molecular marker-assisted breeding for Phalaenopsis with complicate genome in the future.

Orchid; Genetic; Quantitative trait; Gene locus; Molecular mapping