Yanling Liu^1,3 , Jinjin Dong¹ , Tingting Yang¹ , Meng Li^1,3 , Guo Yang¹ , Zhi Feng¹ , Peng Zhan¹ , Guibin Wang² , Wanwen Yu² , Yiqiang Wang^1,3

1 Hunan Provincial Key Laboratory for Forestry Biotechnology, Central South University of Forestry and Technology, Changsha, Hunan, 410004, P.R. China
2 Co-innovation Center for the Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing, P.R. China
3 Key Lab of Non-wood Forest Nurturing and Protection of National Ministry of Education, Central South University of Forestry and Technology, Changsha, P.R. China
Author    Correspondence author
Tree Genetics and Molecular Breeding, 2021, Vol. 11, No. 2   doi: 10.5376/tgmb.2021.11.0002
Received: 29 Sep., 2021    Accepted: 12 Oct., 2021    Published: 28 Oct., 2021

This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Liu Y.L., Dong J.J., Yang T.T., Li M., Yang G., Feng Z., Zhan P., Wang G.B., Yu W.W., and Wang Y.Q., 2021, Cloning and sequence analysis of GBM5 gene from Ginkgo biloba, Tree Genetics and Molecular Breeding, 11(2): 1-9 (doi: 10.5376/tgmb.2021.11.0002)

AGAMOUS (AG), a member of MADS-box gene family, is a key gene related to regulation of flower development in plants. The AG homologous gene GBM5 (Accession No.: AY114304.1) was obtained from the transcriptome of Ginkgo biloba. Primers were designed according to the obtained GBM5 gene sequence, and the full-length cDNA sequence of GBM5 gene with 732 bp in size and 243 amino acids in coding product can be synthetized by designing primers. The comparative analysis of evolutionary tree and amino acid sequence showed that ginkgo GBM5 was closely related to cycads AG and black spruce AG. qPCR analysis showed that the expression of GBM5 in different tissues of Ginkgo biloba was different at different periods, the expression levels of different tissues are as follows: Female flower>Male flower>Primordia>Foliage; Its expression is high in the undifferentiated stage of flower bud, the expression patterns were: April leaf>May leaf>September leaf, April primordia>May primordia>September primordia.

Ginkgo biloba; AG gene; Flowering regulation; Expression analysis