Purity Identification of Chemical Emasculation Hybrid Rape (Brassica napus) Qinyou 88 with InDel Markers
1 Hybrid Research Center of Shaanxi Province, Shaanxi Rapeseed Branch of National Centre for Oil Crops Genetic Improvement, Yangling, 712100, China
2 College of Plant Science and Technology, Huazhong Agricultural University, Wuhan, 430070, China
Molecular Plant Breeding, 2022, Vol. 13, No. 21 doi: 10.5376/mpb.2022.13.0021
Received: 12 Jul., 2022 Accepted: 18 Jul., 2022 Published: 26 Jul., 2022
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This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Li B.J., Shang L.P., Wang Z.Y., Zhang L.J., Guo K.H., Zhao X.P., Zhao W.G., Li D.R., Zhang C.Y., and Wang H., 2022, Purity identification of chemical emasculation hybrid rape (Brassica napus) Qinyou 88 with InDel markers, Molecular Plant Breeding, 13(21): 1-8 (doi: 10.5376/mpb.2022.13.0021)
InDel (insertion deletion length polymorphism) markers is designed for PCR detection based on the Insertion or deletion of DNA fragments of different sizes according to the sequence of a locus between different individuals of related species, which is more and more applied in crop genetic research due to its rich polymorphism, operational simplicity and reliability of results. In this study, Qinyou 88 and the parents were analyzed through InDel marker, and four pairs of candidate primers were obtained to identify the seed purity of Qinyou 88 with complementary band type and stable amplification results. Two pairs of primers ID 2 and ID 15 were verified through identified the high purity hybrids and parents, and the consistency reached 95.5%, 100% and 100%; 97.5%, 99.5 and 100%, respectively. By comparing field identification results with molecular marker identification results, the average identification results of ID 2 and ID 15 primers and field identification results were 96.14% and 95.98%, nevertheless, the analysis deviation between the two pairs of primers was only 0.84. The consistency rate between the molecular identification results and field identification results and the two pairs of primers was very high, reaching an extremely significant level. The results show that it is feasible and reliable to analyze the seed purity of Qinyou 88 using InDel molecular marker.
Brassica napus; Qinyou 88; Insertion deletion length polymorphism; Purity identification