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Association Analysis of Resistance to Soybean Mosaic Virus and Variation in Resistance Allelic Excavation

Soybean Mosaic Virus (SMV) is one of the most important soybean diseases in the world, and it seriously affects soybean yield and quality. In this paper, 405 SSR molecular markers were used in genome-wide scan to analysis 327 soybean varieties in the North of China. The purpose was to search ... (11 Apr., 2021)

Construction of Plant Regeneration System of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang

In order to establish the plant regeneration system of Glycyrrhiza mongolicum and Glycyrrhiza Xinjiang, this study used hypocotyls, leaves, cotyledons and stems to inducing callus and regenerated plant, and it screen out the most suitable explants and the best medium f... (29 Jan., 2021)

Differential Expression Analysis of Genes Related to Flesh Color in Hylocereu polyrhizus and Hylocereu undatus

Pitaya is a burgeoningly tropical fruit, there are two common varieties, red peel with red pulp and red peel with white pulp. To investigate the reason of different colors in two kinds of pitaya, explore differencially expressed genes (DEGs), in this study, we profiled transcriptome in flesh o... (19 Feb., 2021)

Development and Application of Pi-k^h Co-dominant Marker for Rice Blast Resistance Gene

Rice, as one of the staple food crops in the world, is reduced by 10~30% every year due to the damage of rice blast. However, the application of marker assistant selection (MAS) with rice disease resistance genes can be effectively, accurately and rapidly used to breed rice blast resistan... (26 Jan., 2021)

A Preliminary Study on Somatic Cell Embryogenesis of Quercus mongolica

In order to establish a callus induction and somatic embryogenesis system of Quercus mongolica , and provide an effective culture system for molecular mechanism research of somatic embryogenesis, the leaves of annual seedlings of Quercus mongolica were used as explants to stu... (04 Dec., 2020)

RNA-Seq Reveals Transcription Factors Involved in Temperature-mediated Anthocyanin Accumulation and Biosynthesis in Purple Pakchoi (Brassica campestris ssp. Makino.)

Temperature is a main environmental factor that affects anthocyanin biosynthesis and accumulation in purple pakchoi (Brassica campestris ssp. Chinensis Makino.). Purple pakchoi is one of the most popular vegetables with high content of anthocyanin in China. Recently, we found that the purple c... (21 Dec., 2020)

Comparison of Expression Activity of Key Genes in Strawberry Fruit Development

Key genes in strawberry fruit development play an important role in the development and maturation of strawberry fruit, and are also important in molecular breeding and genetic improvement. UPL probes was used to quantitatively analyze the expression of strawberry genes FaNCED1 , FaPG , FaE... (18 Sep., 2020)

Construction and Verification of CRISPR/Cas9 Gene Editing Vector for Cassava MeSSIII Gene

Starch glucan chain structure of cassava root is the key factor to determine starch quality. Soluble starch synthase III (SSIII) is the key enzyme to regulate the synthesis of long chain in plant amylopectin glucan. Cassava has two MeSSIII homologous genes MeSSIII-1 and MeSSIII-2 . To stu... (04 Sep., 2020)

Quantitative Trait Locus Locating Analysis of Bud Set time in an F1 Hybrid Population of Populus deltoides and Populus simonii

Growth and dormancy are the two most important biological processes in the life cycle of perennial plants. Studying dormancy-related traits is of great significance for understanding the adaptability of forest growth and improving the efficiency of plant molecular breeding. In order to reveal ... (14 Aug., 2020)

Validation of Housekeeping Genes as Internal Control for Studying Gene Expression in Rhododendron molle G. Don Located on Dabie Mountains

Rhododendron molle G. Don, a member of the family of Rhododendron, possesses high ornamental and medicinal value. As a species specific to Dabie Mountains, its distribution was scattered. The estimation of expression stability of internal reference genes was vital for molecular biological an... (07 Aug., 2020)

Screening and Identification of NsylCBL Family Members Interacting with Protein Kinase NsylCIPK24a in Nicotiana Sylvestris

The CIPK (CBL-interacting protein kinase) kinase family in plants is a type of serine/threonine protein kinase family. The members of this family interact with the upstream CBL protein (Calcineurin B-like protein) to form the CBL-CIPK signal system, involved in regulating plant growth and deve... (19 Jun., 2020)

Characterization of Genomic Microsatellite Markers and Analysis of Pollen Donors Number for Single Cone of Chinese fir

Chinese fir [ Cunninghamia lanceolate (Lamb.) Hook] is one of the most important indigenous timber tree species in China. The aim of the present work is to characterize simple sequence repeat (SSR) loci derived from the specific length amplified fragment sequencing (SLAF) data of the genome a... (14 Aug., 2020)

Identification and Evaluation of the Expansin Genes in Salix purpurea Genome

Expansin is an indispensable cell wall protein, involved in plant growth and resistance to stress. Based on the sequencing genome data, a total of 34 expansin genes were identified on 14 chromosomes in Salix purpurea , which were grouped into 4 subfamilies. The analysis of the physicochemical... (14 May, 2020)

Development and Application of SSR Makers with Transcriptional Sequencing Data of Female and Male Flowers in Chestnut

In present study, nineteen simple sequence repeat (SSR) loci were isolated and characterized from male and female flower-specific expression genes, which derived from a transcriptional sequencing database of female and male flowers in chestnut. Among these pairs of SSR primers, five pairs show... (28 Apr., 2020)

Principle and Strategy of DNA Fingerprint Identification of Plant Variety

Plant variety identification has profound meanings to ensure seed quality and food safety. DNA fingerprinting has its advantage in plant variety identification, and has transformed from indirect evidence into a mainstream method. A method for DNA fingerprinting called core loci combination was... (11 Aug., 2019)

Molecular Cloning and Expression Analysis of C Function Gene CjPLE in Double Flower Varieties of Camellia japonica

The C function gene in the classic ABC model is the important factor that determines the stamen and pistil organs in the floral axis, and is also the regulatory gene of floral meristem termination differentiation. A 901 bp cDNA sequence of C function genewas cloned from flower bud of Camelli... (03 Aug., 2019)

Genome-wide Identification and Bio-informatics Analysis of Superoxide Dismutase Gene Family in Zea mays

Superoxide dismutase (SOD) is an important antioxidant enzyme and widely exists in organisms. The whole genome scanning and bioinformatics analysis of millet superoxide dismutase (SOD) gene family were carried out by using bioinformatics method on the search platform of maize gene database. Th... (15 Jul., 2019)

Construction of Fingerprint of Michelia Germplasm by Fluorescent SSR Markers

In this study, a fingerprinting system for 16 Michelia germplasms was established using high-throughput genotyping of fluorescent simple sequence repeat (SSR) markers. In total, 102 alleles were detected using 13 pairs of SSR markers from closely related species, and the number of allel... (23 May, 2019)

QTLs Location of Calyx-related Traits in Tetraploid Dendrobium

In order to identify QTL locus of calyx-related traits in Dendrobium, 190 F1 individuals derived from the crossbreed of tetraploid Dendrobium cultivars D. Mangosteen x D. Burana Pink No.2 were used as materials. Through three years observation of four characteristics of calyx (length of dors... (29 Mar., 2019)

Identification and Mapping of a Blast Disease Resistance Gene in Rice Line IR65482

Rice line IR65482 has broad-spectrum resistance to Magnaporthe oryzae from different areas, and it has been known that there is a blast resistance gene Pi40 ( t ) on its chromosome 6. In the present study, using a bulked segregant analysis sequencing strategy, we identified another rice bl... (30 Aug., 2019)