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Tibet Development and Breeding Application of the Fluorescence Molecular Marker of Rice Bacterial Blight Resistance Gene Xa7

In this study, the closely linked fluorescence molecular marker PM-Xa7 of Xa7 gene was developed on the basis of sequence difference between the resistant variety ‘IRBB7’ containing Xa7 and other nine bacterial blight susceptible rice varieties, and combine... (25 Jul., 2021)

Cloning and Expression Analysis of a Salt-Stress-Induced HD-Zip Transcription Factor HB-12 from Sunflower (Helianthus annuus L.)

HB transcription factor genes play a significant role in plant growth and development, including response to biotic and abiotic stresses. In this study, an HD-Zip transcription factor HB-12 was cloned from sunflower using SMARTer RACE technology based on Unigene551_All known... (15 Jul., 2021)

Application of SRAP and CDDP Markers in Genetic Diversity Analysis of Morinda officinalis How.

The analysis of the main germ plasm resources of Morinda officinalis How. in Zhaoqing area and the definition of the relationship among the varieties will provide scientific basis for the cultivation, protection and identification of Morinda officinalis How.. SRAP and C... (30 Jun., 2021)

The Regulation of Brassinolide Biosynthesis and Physiological Effects in Grape Berries

Brassinolide is an important plant hormone regulating plant growth and development, promoting cell division and elongation, photosynthesis and respiration, photomorphogenesis, stress resistance improvement, transpiration reduction and make a very important contribution to the improvement of gr... (30 Oct., 2021)

Identification and Bioinformatics Analysis of KNOX Gene Family in Wheat (Triticum aestivum L.)

KNOTTED-like homeodomain ( KNOX ) gene family is a transcription factor encoding homeobox protein, which plays an important role in plant growth and morphogenesis. However, little information is available on the KNOX gene family in wheat ( Triticum aestivum L.). In this study... (20 Jun., 2021)

Cloning and Expression Analysis of PAP1 in Brassica juncea

Anthocyanins are important substances accounting for the leaf color in Brassica juncea and PAP1 gene is one of the key transport factors in the anthocyanin synthesis pathway. In this study, homologous cloning technology was used to clone the PAP1 gene sequences of Brassica jun... (02 Jun., 2021)

Tibet The Seed Germination Characteristics Under Low Temperature and Spring Sowing Study of 8 Rapeseed Varieties

To explore the low temperature germination characteristics of rapeseed, 8 main cultivars were selected for germination experiments in incubators at 20℃, 16℃, 12℃ and 8℃, respectively, to measure and analyze the germination potential, germination rate, root length, bud length, etc. The results ... (25 May, 2021)

Sequencing of Chloroplast Genome of Cucumis melo L. var. agrestis Naud.

Cucumis melo L. var . agrestis Naud. belong to the genus Cucumis in the Gourd family, it is rich in oil. It can extract high quality edible oil and provide raw materials for medicine and food industry. The chloroplast genome of Cucumis melo L. was sequ... (25 May, 2021)

The Complete Chloroplast Genome of a Variety of Lagenaria siceraria var. microcarpa (Naud.) Hara

Small gourd ( Lagenaria siceraria var . microcarpa (Naud.) Hara), a cultivar of Lagenaria family, is an important medicinal and ornamental plants. However, there are few studies on its chloroplast (cp) genome. In this study, we characterized its complete chloropl... (16 Jul., 2021)

Comparative Transcriptome Analysis of ‘GR2’ and ‘GL05136’ in Mature-stage of Sugarcane

Sugarcane 'GR2' and 'GL05136' belong to late and early maturing high sugar varieties. Transcriptome sequencing was performed on the mixed samples of the leaves and stems of the two varieties to compare the differences in gene expression and to screen and clone genes that may be... (20 May, 2021)

Identification of Seed Purity on Hybrid Japonica Rice ‘Huayou 14’ Using SSR Markers

In this study, the hybrid japonica rice ‘Huayou 14’ and its parents were used as materials, using SSR molecular marker technology, to select primers and identify seed purity according to its polymorphism. From 48 pairs of candidate SSR molecular markers widely distributed on 12 ric... (03 Sep., 2021)

Screening and Bioinformatics Analysis of Proteins Interacting with StMAPKK1 in Potato by Yeast Two-Hybrid System

Mitogen-activated protein kinase (MAPK) cascade reaction is one of important and complex signal networks involved in plant growth and development, hormone and stress response. As one of its main members, mitogen-activated protein kinase kinase (MAPKK) is located in the middle of the cascade re... (31 Mar., 2021)

Genetic Diversity and Population Structure of Chinese Jujube (Ziziphus jujuba Mill.) and Sour Jujube (Ziziphus acidojujuba Mill.) using Inter-simple Sequence Repeat (ISSR) Markers

The Chinese jujube ( Ziziphus jujuba Mill.) originates from sour jujube ( Ziziphus acidojujuba Mill.) and is an economically important genus in the Rhamnaceae family. However, little is known about the genetic relationship between jujube cultivars and wild species. In this study, w... (20 May, 2021)

Effect of Activated Carbon on Rooting of Tissue Culture Seedlings of Qiuzi Pear

In order to improve the rooting rate of Qiuzi pear tissue culture seedlings, this study took 'sorb' as experimental material, based on Qiuzi pear rooting tissue culture medium (1/2MS+IBA 0.5 mg/L), and added different concentrations of activated carbon (AC): 0.25 g/L, 0.50 g/L, 0.75 g/... (25 Mar., 2021)

Genetic Diversity and Relationship Analysis of 21 Walnut Varieties Based on SSR Markers

In order to grasp the germplasm resources of walnut in Yunnan province, the genetic diversity and relationship of 21 walnuts varieties commonly planted in Yunnan province were analyzed using SSR fluorescent labeling technology. The results showed that the average number of allelic loci of 12 ... (31 Mar., 2021)

Identification and Analysis of PGM and SUS Gene Families in Leymus Chinensis Seed Transcriptome

Leymus chinensis ( Leymus chinensis (Trin.) Tzvel.) is a good perennial forage with good palatability, high yield and high feeding value. In addition, because of its strong resistance, Leymus chinensis can improve the disadvantaged environment such as poor land, and it is an importa... (19 Feb., 2021)

The Development of EST-SSR Markers from Transcriptome Sequencing and Genetic Diversity of Twenty Genotypes with High Yields of Cornus wilsoniana, an Important Wood Oil Plant

Oil plants are not only used as essential nutriments in food but also used broadly as ingredients of industrial products. Cornus wilsoniana Wanger is an important native woody oil plant with high-yield and high-oiliness characters in China. The fruit oil of Cornus wilsoniana&... (07 Apr., 2021)

Identification of Ploidy Variation of Ponkan Embryo Rescue Plants

China has abundant of local citrus resources, which had many seeds. The Seedless is the aim of the local citrus breeding. Triploid citurs resources usually obtained by crosssing breeding between the diploid and tetraploid plants. However, the resources of tetraploid citrus are limited. It has ... (31 Dec., 2020)

Cloning, Spatiotemporal Expression and Prokaryotic Expression of ACC Oxidase Gene (BnACO2) in Ramie

ACC oxidase (ACO) is a key enzyme in ethylene synthesis pathway. This study by rt-pcr with RACE technology get a ACO gene cloning, named BnACO2 , total length of the gene cDNA sequence is 1 377 bp, the open reading frame of 957 bp, coded 318 amino acid polypeptide, predict its molecular ... (15 Jan., 2021)

Bioinformatics Analysis of Cellulose Synthase CesA Gene from Miscanthus lutarioriparius

Based on 3 generations transcriptome sequencing technology-PacBio SMRT single-molecule real-time sequencing method, supplemented with 2 generation sequencing method, a complete and accurate unigene library was constructed. After comparing with closely related species, the cellulose synth... (22 Jan., 2021)