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Construction of Overexpression Vector of CONSTANS Gene Plant in Brassica napus and Production of Transgenic Plants

The CONSTANS target gene from Brassica napus was cloned, and the full length amplified primer of it was designed and amplified on the sequence released on NCBI. The target gene fragments were sequenced and the restriction loci were analyzed. The enzyme primers (BamHⅠ, SacⅠ) were designe... (14 Dec., 2018)

Identification and Detection Analysis of SSR Purities of Three Tender Pumpkin Cultivars

Simple sequence repeat (SSR) molecular markers and traditional field identification were used to identify the purity of three widely popularized hybrid cultivars of tender pumpkin, named Nenzao 2, Nenzao Jiamei and Nenzao Heiniu. It turned out that primer NG05, NG74 and NG88 all amplified spec... (30 Oct., 2018)

Allele-specific PCR Markers for Distinguishing High Molecular Weight Glutenin Subunit Dtx5 of Aegilops tauschii from Dx5 of Common Wheat

High molecular weight (HMW) glutenin, one of the important storage proteins of wheat, mainly confers end-use quality of wheat grain. Among the loci of HMW-GS, the locus D1 determines the breaking quality of bread wheat dough mostly. Synthetic hexaploid wheat (SHW) inherits vast genetic diversi... (20 Jul., 2018)

Hybridization Breeding between Triploid OT Lily and Diploid Oriental Lily

This study was aimed to find out the law of hybridization breeding between triploid OT lily and diploid O lily, and breed interspecific hybrids. The conventional compression method was used to analyze maternal chromosome karyotypes. The cut style and normal stigma pollination methods were appl... (04 May, 2018)

Whole Genome-wide Identification of Homologous Candidate Genes in Legume Related to the Regulation of Circadian clock

In this study, we employed comparative genomics and gene ontology theory, and used genome database of Glycine max and its legume model plants Medicago truncatula , Lotus Japonicus , Glycine max to identify 11 candidate genes, ELF3/ELF4 , CCR2, CRB , ... (31 Mar., 2017)

Identification of Genes Related to Photoperiod that Regulating the Circadian Clock in Legume Plants

In this study, 9 kinds of genes related to photoperiod-mediated circadian clock were analyzed by bioinformatics methods in order to identify the genes related to photoperiod that regulate circadian clock in legume, which were Phytochrome, Cryptochrome, LKP1/FKFI/LKP2, PIF3/PIF4/PIF5,... (30 Mar., 2017)

Diversity Analysis of Forty-one Tree Peony Cultivars by EST-SSR Markers

Tree peony and related industries make up quite a large proportion in tourism income of Luoyang, China. Short flowering feature of most native tree peony cultivars is a key restrictive factor of economic benefits increasing. Hybridizing native superior cultivars with late-flowering introduced ... (03 Mar., 2017)

Mapping of Nuclear Male-Sterile Gene ms₁₄ Using SSR Markers in Cotton

Nuclear male sterility (NMS) is a very important character for cotton breeding and genetics programs. Identification of linked molecular markers with NMS will greatly facilitate breeding for this trait. Among all of the Nuclear male-sterile genes, only Dong-A ( ms 14 ) was utilized successful... (23 Dec., 2016)

Insight in the utilization of Marker Assisted Selection in Cotton (A Review)

Upland cotton represents the most important, and natural fiber crop in the world. Limitations in conventional breeding program for genetic improvement is due to the lack... (30 Mar., 2016)

Differential expressed genes of banana leaves induced by Fusarium wilt disease

Fusarium wilt of banana is a destructive fungus disease caused by Fusarium oxysporum f. sp. Cubense . This disease had harmful effects on banana planting and its industrial production. In order to study the pathogenic mechanism of banana Fusarium wilt disease, we used cDNA-AFLP technique to id... (08 Nov., 2015)

Development of new set of microsatellite markers in cultivated tobacco and their transferability in other Nicotiana spp.

Scarcity of molecular markers in tobacco has been a limitation, hampering the acceleration of breeding efforts. Development of microsatellite markers is a prerequisite for mapping, tagging of many useful qualitative and quantitative traits and also for the generation of saturated linkage map. Use... (14 Oct., 2015)

Development of SCAR Marker Linked to Sex Determination Locus in Trichosanthes dioica

Pointed gourd ( Trichosanthes dioica Roxb .), a perennial herb of family Cucurbitaceae, is morphologically different from the other cucurbits due to its dioecism and vegetative means of propagation. Breeding in pointed gourd is less viable due to poor germination, vegetative propagation mean and ... (13 Oct., 2015)

Biosorption Property of Cadmium (II) in Marsilea minuta L. with Polyamine Interaction

A study was undertaken for biosorption following bio accumulation property of Marsilea minuta L., an aquatic pteridophyte in cadmium enriched solutions. The possible mechanism for biosorption on the cell wall residues has been discussed with exogenous applications of polyamine. Initially plants... (08 Oct., 2015)

Genetic diversity analysis in tropical maize germplasm for stem borer and storage pest resistance using molecular markers and phenotypic traits

One hundred maize inbred lines and eighty four hybrids were characterized for resistance to maize stem borer and post-harvest insect pests. This was achieved using genetic distance and population structure based on simple sequence repeat (SSR) markers and biophysical traits. The test materials we... (16 Oct., 2015)

DNA Barcoding for Wild Rice [Oryza rufipogon Griff.] of NBU Campus Based on matK gene and Assessment of Genetic Variation Using DREB and BAD2 Gene Sequences

DNA barcoding is a technique for characterizing species based on short DNA sequence of a particular genomic region. The chloroplast maturase gene K (matK) sequence of 1420 bp was used to construct unique DNA barcode for wild rice [ Oryza rufipogon Grirr.] of NBU campus using BOLD system. The par... (17 Jul., 2015)

Assessment of genetic divergence in Chilli (Capsicum annuum L.) genotypes

Thirteen genotypes of chilli ( Capsicum annuum L.) were investigated to understand the extent of genetic diversity through 6 yield attributing characters. Genetic diversity in chilli genotypes based on six characters was estimated using Mahalanobis’s D 2 statistics. The genotypes were gr... (04 May, 2015)

Genome-wide identification and characterization of heat shock factor genes from pigeonpea (Cajanus cajan)

Genome-wide analysis of heat shock factor ( H s f ) g e n e s was ca r r ied out in p i g e on p e a ( Cajanus cajan ) in order to understand their structure and function. A tot a l of 23 Hsfs w e r e p re dict e d using FGENESH a nd l a b e led a s C c Hsf . Out of the 2 3 ... (26 Mar., 2015)

The feasibility of QTL analysis to study the genetic basis of pesticide residues in rice (Oryza sativa)

Pesticide residues in Chinese rice have become one of the important issues as far as food safety is concerned. Although QTL mapping and cloning are important tools for molecular genetics, not much information has been reported yet for either QTL mapping or cloning the genes of rice pesticide deg... (29 Sep., 2014)

Mapping QTLs Related to Salt Tolerance in Rice at the Young Seedling Stage using 384-plex Single Nucleotide Polymorphism SNP, Marker Sets

Salinity is one of the most serious factors limiting the productivity of rice crops, with adverse effects on germination, plant vigor and crop yield. A population of 300 F 5:6 recombinant inbred lines (RILs), which was derived from a cross between IR29 (indica sensitive to salinity) and Hasawi (... (13 Jun., 2014)

Microsatellites Based Genetic Linkage Map of the Rht3 Locus in Bread Wheat

The wheat reduced height genes ( Rht ) played an important role in “The Green Revolution” by reducing damage due to lodging and making wheat more responsive to fertilizer applications. The successful use of cultivars containing Rht-B1b and Rht-D1b around the world compelled the sc... (12 Jun., 2014)